Mohammad Jafar Emami; Fereidoon Mojtahed Jaberi; Negar Azarpira; Amir Reza Vosoughi; Nader Tanideh, DVM, MPH, PhD
Abstract
Background: Prevention of arthrofibrosis by different drugs and surgical techniques is an essential issue in modern orthopaedics. This study investigated the effect of bevacizumab on intra-articular fibrosis in a rabbit animal model.Methods: Arthrofibrosis was induced in right stifle joint of thirty ...
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Background: Prevention of arthrofibrosis by different drugs and surgical techniques is an essential issue in modern orthopaedics. This study investigated the effect of bevacizumab on intra-articular fibrosis in a rabbit animal model.Methods: Arthrofibrosis was induced in right stifle joint of thirty male white rabbits by removing the cortical bone of femoral condyle in medial gutter under general anesthesia. The rabbits were randomly divided into three equal groups. The control group received intra-articular injection of saline the "one-injection" group received a single dose of bevacizumab (2.5 mg/kg), and the" two-injection" group received two intra-articular injections on the day of operation and 14 days later. Forty five days after the surgery, the animals were sacrificed. The severity of fibrosis was assessed by range of motion of the stifles, macroscopic adhesion score, and histopathologic variables.Results: Although no statistical difference was seen between the control group and the "one-injection" group in terms of range of motion and macroscopic evaluation, the histopathologic variables in prevention of arthrofibrosis, except for the granulation tissue, were significantly better in the "one-injection" group. The "two-injection" group in comparison to the "one-injection" group was better not only in range of motion evaluation (p < /em>=.001) and visual adhesion score (p < /em>=.012), but also in most of the histopathologic variables, except for the cell count and granulation tissue.Conclusions: Two injections of bevacizumab for prevention of arthrofibrosis resulted in better outcome than single injection.
Zahra Hassanzadeh Rostami, MSc; Mohammad Hassan Eftekhari, Ph.D; Mohammad Jafar Emami, MD; Abdolreza Rajaeefard, Ph.D
Abstract
Background: Phytoestrogens enhance bone integrity
through prevention of bone loss and stimulation of bone formation, and also facilitation
of bone healing. The aim of the present study was to evaluate the effect of vitex agnus castus extract, as a source of phytoestrogen on alkaline phosphatase and
VEGF ...
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Background: Phytoestrogens enhance bone integrity
through prevention of bone loss and stimulation of bone formation, and also facilitation
of bone healing. The aim of the present study was to evaluate the effect of vitex agnus castus extract, as a source of phytoestrogen on alkaline phosphatase and
VEGF (vascular endothelial growth factor) and also callus formation in women
with long bone fractures. Methods: In a double-blind randomized placebo
controlled trial, 32 women with long bone fracture, age 20-45 years old, were
randomly divided into the VAC (vitex agnus
castus), receiving 1 Agnugol tablet (containing 4 mg dried fruit
extract of vitex agnus castus)-daily for 8 weeks or a placebo group. Serum alkaline
phosphatase and VEGF were measured at the beginning and the end point, and also
callus formation was checked at the end of study. Results: Serum alkaline phosphatase was increased
in both groups compared to the baseline. Although the elevation of ALP
(Alkaline phosphatase) in placebo group was not significant, in treatment
group, ALP was considerably increased (p=.05). Comparison of the change of VEGF
showed that, only in VAC group, VEGF level was significantly increased (p < /i>=.01).
The callus formation which revealed the fracture healing, in VAC and control
groups was 71.4% and 53.8%, respectively. Conclusions: Treatment with vitex agnus castus extract for 2 months could enhance the VEGF
level, however, further studies are need to show the effects of vitex agnus castus extract on alkaline phosphatase
level and callus formation.
